繁殖生理與細胞生物學 (Reproductive Physiology and Cell Biology) 研究室

主持人:蔡沛學 助理教授

人事現況:本研究室為2014年4月開始設立,歡迎生物醫學相關科系(獸醫與非獸醫學系均可)並對繁殖生理 (Reproductive Physiology) 與细胞生物學 (Cell Biology)有興趣之同學加入本研究室。



簡介、研究現況:

蔡沛學助理教授2000年畢業於中興大學獸醫學系,並取得獸醫師執照與通過專技高考。2002-2010年於荷蘭烏特列支大學 (Utrecht University)生化與細胞生物學系先後取得碩、博士學位。碩士期間以流行病學角度,探討 DNA deficiency 對繁殖之影響;並於 Dr. Bart Gadella 實驗室完成博士論文 (2010)。研究主題為細胞膜蛋白動力學(Membrane protein dynamics)於生殖醫學領域之角色,尤其專注於哺乳類 SNARE 蛋白在生殖細胞膜融合 (membrane fusion) 之作用機轉。

2010-2014年間,蔡沛學助理教授先後於荷蘭烏特列支大學 (with Dr. Bart Gadella) 、美國麻州大學醫學院 (with Dr. Harvey Florman) 與哈佛醫學院 (with Dr. Dennis Brown and Dr. HA Jenny Lu) 進行博士後研究。主題延續膜蛋白動力學之基礎醫學研究及應用。

本研究室研究重點將致力於建構細胞膜蛋白動力學研究之實驗動物模型,以期提供不孕症與特定疾病之檢測或治療應用契機。
研究方向主要包括:
(一) 精子熟成過程 (sperm maturation) 之分子調控機制。
(二) 細胞膜蛋白 (membrane proteins) 與囊泡傳輸系統 (intracellular vesicle trafficking) 於腎臟病之致病機轉及病程發展的影響。
(三) 细胞骨架 (cytoskeleton) 於細胞膜蛋白動力學之分子調控機制。


近期學術著作:

期刊論文 (Refereed Journals):

  1. H. Florman, WL Cao, MK Jungnickel, KA Sutton, Tsai PS. Coup d’oeil of Sperm. Molecular Reproduction and Development 2012, 79(5) 22039 (SCI: IF 2.81).

  2. Tsai PS, Brewis IA, J. Van Maaren, Gadella BM. Involvement Of Complexin 2 in Docking, Locking and Unlocking of diffeent SNARE complexes in induced Acrosome Exocytosis. PLoS ONE 2012, 84:327-335 (SCI: IF 4.41).

  3. Tsai PS, Van Haeften T, Gadella BM. Preparation of the cortical reaction: maturation dependent migration of SNARE proteins, clathrin and complexin to the porcine oocyte’s surface blocks membrane traffic until fertilization. Biology of Reproduction 2011, 84:327-335 (SCI: IF 4.14).

  4. Tsai PS, Garcia-Gil N, Colenbrander B, Van Haeften T, Gadella BM. How pig sperm prepares to fertilize: acrosome docking and priming to the plasma membrane. PLoS ONE 2010 Jun 18;5(6):e11204 (SCI: IF 4.41).

  5. Tsai PS, Gadella BM. Molecular kinetics of proteins at the surface of porcine sperm before and during fertilization. Soc. Reprod. Fertil. Suppl. 2009;66:23-36. Review. (SCI: IF 2.5).

  6. Gadella BM, Tsai PS, Boerke A, Brewis IA. Sperm head membrane reorganisation during capacitation. International Journal of Developmental Biology 2008;52(5-6):473-80. Review. (SCI: IF 2.61).

  7. Boerke A, Tsai PS, Garcia-Gil N, Brewis IA, Gadella BM. Capacitation-dependent reorganization of microdomains in the apical sperm head plasma membrane Functional relationship with zona binding and the zona-induced acrosome reaction. Theriogenology 2008 Nov;70(8):1188-96. Review. (SCI: IF 2.08)

  8. Tsai PS, De Vries KJ, De Boer-Brouwer M, Garcia-Gil N, Van Gestel RA, Colenbrander B, Gadella BM, Van Haeften T. Syntaxin and VAMP association with lipid rafts depends on cholesterol depletion in capacitating sperm cells. Molecular Membrane Biology 2007 Jul-Aug;24(4):313-24. (SCI, IF 3.6)

  9. Wu JT, Tsai PS, Lee SL, Cheng FP. Characterisation of the progesterone receptor on canine spermatozoa. Reprod. Fertil. Dev. 2005 17(7):733-741. (SCI, IF 1.52)

  10. Tsai PS, Nielen M., van der Horst GTJ, Colenbrander B., Heesterbeek JAP, Fentener van Vlissingen JM. The effect of DNA repair defects on reproductive performance in nucleotide excision repair (NER) mouse models: An epidemiological approach. Transgenic Research 2005 14:845-857. (SCI, IF 2.54)

  11. Cheng FP, Wu JT, Tsai PS, Chang CL, Lee SL, Lee WM, Fazeli A. Effects of cryo-injury on progesterone receptor(s) of canine spermatozoa and its response to progesterone. Theriogenology 2005 64(4):844-854. (SCI, IF 2.16)

書籍著作 (Book Chapter):

  1. Tsai PS, Gadella BM. Molecular kinetics of proteins at the surface of porcine sperm before and during fertilization. Chapter 2, Control of Pig Reproduction VIII, 2009. Nottingham University Press. ISBN: 978-1-904761-39-6

研討會論文 (Proceedings):

Oral Presentation

  1. Tsai PS, Gadella BM. Annual meeting of the Institute of Biomembranes. 28th-29th October 2010. Utrecht, the Netherlands. Stable docking of the acrosome to the sperm plasma membrane is a preparative step required for the mammalian fertilization (Invited presentation)

  2. Tsai PS, Gadella BM, Helms B. NWO-CW Annual meeting for Proteins, Lipids, Nucleus and Biomembranes. 7th-9th December 2009. Veldhoven, the Netherlands.

  3. Bart Gadella, Tsai PS. VIII Annual meeting for the Society of Reproduction and Fertility (SRF, ICPR). 31st May-4th June 2009. Banff, Canada. Molecular kinetics of proteins at the surface of porcine sperm before and during fertilization (Invited presentation).

  4. Tsai PS. Annual meeting for Dutch Veterinarian Science Day. November 22nd 2007. Zeist, the Netherlands. Capacitation-dependent formation of a SNARE protein complex involved in the acrosome reaction.

  5. Tsai PS. 40th Annual meeting for Society for the Study of Reproduction (SSR). July 21st-25th 2007. San Antonio, Texas, USA. SNARE redistribution during capacitation depends on association with lipid rafts.

  6. Tsai PS. NWO-CW Annual meeting for Lipids and Biomembranes. March 5th- 6th 2007.  Lunteren, the Netherlands. Syntaxin and VAMP association with lipid rafts depends on cholesterol depletion in capacitating sperm cells.

Abstract and poster presentations

  1. P.S. Tsai, IA Brewis, B.M. Gadella Gordon Research Conference (Fertilization, Activation and Development). July 17th-22nd 2011. Holdness, NH, USA. How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.

  2. P.S. Tsai, N. Garcia-Gil, T. van Haeften, B.M. Gadella NWO-CW Annual meeting for Lipids and Biomembranes. December 6th-7th 2010. Veldhoven, the Netherlands. How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.

  3. P.S. Tsai, T. van Haeften, B.M. Gadella NWO-CW Annual meeting for Lipids and Biomembranes. December 6th-7th 2010. Veldhoven, the Netherlands. Preparation of the cortical reaction: maturation dependent migration of SNARE proteins, clathrin and complexin to the porcine oocyte’s surface blocks membrane traffic until fertilization.

  4. P.S. Tsai, A. Boerke, N. Garcia, B. Colenbrander, J.B. Helms, T.A. van Haeften, B.M. Gadella NWO-CW Annual meeting for Lipids and Biomembranes. March 10th-11th 2008. Lunteren, the Netherlands. Capacitation of mammalian sperm leads to SNARE complex formation and docking of the plasma membrane with the acrosome.

  5. Boerke A., P.S. Tsai, G. van der Velde, B. Colenbrander, J.B. Helms, B.M. Gadella. NWO-CW Annual meeting for Lipids and Biomembranes. March 10th-11th 2008. Lunteren, the Netherlands. Capacitation-dependent clustering of sperm membrane microdomains with lipid raft-like features.

  6. Tsai PS. 40th Annual meeting for Society for the Study of Reproduction (SSR). July. 21st-25th 2007. San Antonio, Texas, USA. SNARE redistribution during capacitation depends on association with lipid rafts.  Biology of Reproduction 2007 77:194–194.

  7. P.S. Tsai, M. Nielen, G.T.J. van der Horst, B. Colenbrander, J.A.P. Heesterbek& J.M. Fentener van Vlissingen. 10th FELASA symposium. June 11th-14th 2007, Villa Erba,. Cernobbio (Como), Italy. Mining the Animal data base (AMS) for the evaluation of reproductive performance in mutant mice: an epidemiological approach.

  8. N. Garcia-Gil, P.S. Tsai, A. Boerke, S. Bonet, B.M. Gadella. 8th International Meeting AERA. October 19th- 21st 2006. Murcia, Spain. Effect of Cryopreservation on the Association of Syntaxin 2 to the Lipid Raft. Reprod. in Domest. Anim. 2006 41(2):113

  9. P.S. Tsai, M. de Boer-Brouwer, N. Garcia-Gil, K.J. de Vries, B. Colenbrander, T.A.E. van Haeften, B.M. Gadella. Annual meeting for Dutch Veterinarian Science Day. May 12nd 2006. Zeist, the Netherlands. Syntaxin 2 association to the lipid rafts during cholesterol depletion in sperm cells. Awarded for “Jury Price 2006”.

  10. Tsai PS, Nielen M., van der Horst GTJ, Colenbrander B., Heesterbeek JAP, Fentener van Vlissingen JM. SVEPM Annual Conference. March 29th-April 1st, 2005. Nairn, Scotland. Reproductive performance in a colony of genetically modified mice.

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